Placing a sample in a magnetic field allows for magnetic circular dichroism (MCD) measurements to be obtained. In protein molecules, MCD can be used as a probe to monitor a chromophore’s local environment. Chromophores with large magnetic moments arising from either rotational symmetries (aromatics, porphyrins), unpaired spins (metal complexes), or both (hemes) are sensitive to electronic perturbations and therefore provide information regarding the molecule’s electronic state.

The most versatile approach to orienting macromolecules for LD measurements is the Couette flow system. This system subjects the sample to a constant gradient over the annular gap between an inner quartz cylinder, which is rotating at high speed, and a fixed outer quartz cylinder. The CFC-573 Couette flow cell unit includes a built-in beam condenser that focuses the beam onto the small LD flow cell to maximize light throughput.

The ATS-530 is designed to automatically monitor changes in CD, absorbance and fluorescence as a function of solution pH, chemical denaturant, or exogenous ligands for studies involving protein denaturation or ligand binding. Dual syringes are employed and are each equipped with a valve for automated refilling and flushing during extended runs and for maintaining a constant cell volume. Additionally, the titration measurement program automatically corrects for concentration.

The J-1500 CD spectrometer can be used for spectral measurements into the NIR region using an extended wavelength range light source and detector. In this configuration, the InGaAs detector kit includes a halogen lamp unit and allows for measurement up to 1600 nm.

Samples that are insoluble or may change conformation when in solution have traditionally been difficult to characterize by CD. Diffuse reflectance uses an integrating sphere to effectively analyze these samples. In addition, diffuse transmission measurements of pellets or films is easily accomplished with the same integrating sphere, substantially increasing the collection efficiency for scattering samples and providing a suitable alternative to conventional transmission measurements.

The P-2000 is a configurable high precision digital polarimeter with many options for measurement range, sampling accessories and software applications. The P-2000 digital polarimeter can also be field upgraded as application requirements change. Optional wavelength filters and source lamps cover a wide range of analytical wavelengths from UV-Visible to NIR in addition to the traditional sodium (589nm) lamp used for regulatory compliance.

The OR-4090 chiral detector measures the angle of rotation of plane polarized light caused by optical active isomers and is useful for chiral compounds with no absorption. The OR-4090 uses a high intensity Hg-Xe arc lamp (150W) providing a strong output in the UV-Visible region where the largest optical rotations offer the highest sensitivity. The wide wavelength range from 350-900nm also allows measurement of a wide variety of samples.

The CD-4095 is a unique HPLC CD detector for measuring circular dichroism developed from the market leading J-Series circular dichroism spectropolarimeters. Optically active compounds with a chromophore close to a chiral center may absorb circularly polarized light, which can be detected with excellent sensitivity and selectivity.

The J-1000 Series Circular Dichroism spectrophotometers are specifically designed for high sensitivity measurements in the near- and far-UV regions, for scientists that need confidence in the data from characterization studies of biomolecule structure, function, and stability under a wide variety of experimental conditions.
Unlike some detectors which only provide good signal-to-noise for low absorbing materials, the J-1000 Series circular dichroism spectrophotometers use high dynamic range detectors to obtain high-quality spectra of samples with excellent signal-to-noise, especially for highly absorbing materials found in the far-UV.

The NRS Series of confocal Raman imaging microscopes includes three different optical configurations; the easy to use, yet powerful NRS-4500, with up to three laser wavelengths (wide range, from visible to NIR) including the common dual laser 532/785nm configuration). The sophisticated NRS-5000 Series, with 300mm spectrograph, offering higher spectral and spatial resolution with the use of dual spatial filtration (DSF) and the NRS-7000 Series with 500mm spectrograph and poly-dispersive options. Both the NRS-5000 and NRS-7000 can be configured with up to 8 lasers (9 wavelengths).

Each base unit has two expansion bays that allow an isocratic pump to be transformed into a binary or quaternary gradient solvent delivery system with internal degasser, mixer and optional solvent selection for method development.

The AS-4050 is a 60 sample autosampler for use with conventional HPLC with options for analytical and semi-preparative injection volumes.