Fluorescence Quantum Yield measurements provide information about the efficiency of a molecule’s fluorescence. Fluorescence quantum yield is measured using an integrating sphere to ensure that all sample fluorescence is collected.

Phosphorescence materials are frequently used to increase the quantum efficiency of materials since the radiative decay from both the triplet and singlet states contributes to the light emission. Since typically the lifetime of phosphorescence is long, thermal deactivation occurs by oxygen quenching, solvent movement, and intermolecular collision and the phosphorescence cannot be observed at room temperature. The sample is therefore cooled to liquid nitrogen temperature to slow down the relaxation process.

These cell holders use the Peltier effect to precisely maintain the sample temperature. The cell holders include a stirrer for uniform cell temperature.

Easy to use and accurate, the One-Drop accessory is ideal for low volume measurement of proteins and nucleic acids, and can be used for quantitation or simple spectral measurements. Only a single 5 μL droplet is required for the 1 mm path length cell.

Fluorescence Measurement for solid, films and powder sample

Used with a temperature controlled circulating water bath (not supplied) to maintain a constant sample and reference cell temperature.